Mediators of Inflammation / 2019 / Article / Fig 3

Research Article

miR-221 Alleviates the Ox-LDL-Induced Macrophage Inflammatory Response via the Inhibition of DNMT3b-Mediated NCoR Promoter Methylation

Figure 3

Silencing DNMT3b attenuated ox-LDL-induced macrophage inflammatory responses via increased NCoR. (a, b) DNMT3b mRNA and protein expression was evaluated by qPCR and western blotting after THP-1 cells were transfected with DNMT3b siRNAs for 48 h and induced with PMA for 48 h (). , vs. the NC group. THP-1 cells were transfected with DNMT3b siRNA and NC for 48 h and pretreated with PMA for 48 h. These cells were treated with ox-LDL for 24 h. NF-κB activity (c), IL-6 and TNF-α mRNA expression (d), IL-6 and TNF-α levels in culture supernatant (e), NCoR mRNA levels (f), and protein levels (g) were analyzed using a luciferase assay, qPCR, FCM, and western blotting, respectively (). , vs. the NC group; ^, vs. the ox-LDL/NC h group; #, vs. the DNMT3b siRNA group.

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