Mediators of Inflammation / 2019 / Article / Fig 5

Research Article

miR-221 Alleviates the Ox-LDL-Induced Macrophage Inflammatory Response via the Inhibition of DNMT3b-Mediated NCoR Promoter Methylation

Figure 5

NCoR overexpression inhibited the ox-LDL-induced inflammatory response in macrophages. (a, b) The protein and mRNA expression levels of NCoR were detected by western blotting and qPCR after THP-1-derived macrophages were treated with ox-LDL at the indicated time points (). , vs. the 0 h group; ^, vs. the 24 h group. THP-1 cells were transfected with adv. HA-NCoR for 48 h and treated with PMA for 48 h. (c, d) The protein and mRNA expression levels of NCoR were evaluated after THP-1 cells were transfected with adv. NCoR for 48 h (). , vs. the MOCK group. (e, f) The levels of IL-6 and TNF-α in culture supernatants and mRNA were measured by qPCR and FCM after macrophages were treated with ox-LDL for 24 h (). , vs. the NC group; ^, vs. the ox-LDL/NC group.
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