HPV-16 Infection Is Associated with a High Content of CD39 and CD73 Ectonucleotidases in Cervical Samples from Patients with CIN-1

Figure 4

Hydrolytic activity of CD39 and CD73 solubilized in cervical samples from CIN-1 patients and NDs. Aliquots of 2 μg of total protein obtained from the supernatants of cervical samples from NDs negative for HPV (ND, black bars) and CIN-1 patients, positive either for HPV-16 (HPV-16, white bars) or for coinfection with HPV-16 and other HPV types (HPV-16+CI, diagonal lines), or HPV patients negative for HPV-16 (HPV-16neg, horizontal lines) were incubated in the presence of 5 mM of ATP or AMP and in the presence or absence of POM-I or APCP, specific inhibitors of CD39 and CD73, respectively. After 72 h of incubation, the Ado product of ATP (a) or AMP (b) hydrolysis was evaluated by UPLC. indicates a significant difference () of the Ado product of CIN-1 samples compared to NDs. Data represent three independent experiments.