Lats2-Underexpressing Bone Marrow-Derived Mesenchymal Stem Cells Ameliorate LPS-Induced Acute Lung Injury in Mice

Figure 2

Underexpression of Lats2 promotes the differentiation of BMSCs into ATII cells. (a) Immunofluorescence staining images of lungs in the MSC-shcontrol and MSC-shLats2 groups are presented from six mouse lungs obtained 14 days after LPS challenge. Engrafted BMSCs, ATII cells, or BMSCs that differentiated into ATII cells in the lung are shown as GFP-positive (green), SPC-positive (red), or double-positive (yellow) under fluorescence microscopy, respectively. The nuclei were stained with DAPI (blue; magnification, ×400; scale  μm). The ratio of the count of SPC-positive to the count of GFP-positive BMSCs in randomly selected high-power fields is presented as the deviation (). (b) The protein expression of SPC in the lung tissue was measured using Western blot analysis 14 days after LPS challenge. β-Actin was used as the internal reference, and the results are presented as the deviation (). .

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