Thrombin Induces Secretion of Multiple Cytokines and Expression of Protease-Activated Receptors in Mouse Mast Cell Line

Figure 1

Cell experiment flow diagram. TM: thrombin; HIR: hirudin; SCH79797: PAR1 inhibitor; SP600125: JNK inhibitor; SB203580: P38 MEPK inhibitor; PD98059: ERK1/2 MAPK inhibitor; qPCR: quantitative real-time PCR; ELISA: enzyme-linked immunosorbent assay. Screening trial for deciding the fittest concentration of thrombin. Stimulative trial: thrombin 0.2 U/ml was used to stimulate P815 cells. Inhibited trial: thrombin 0.2 U/ml plus different inhibitors were used to stimulate P815 cells. Blank: P815 cells were cultured in normal condition with no challenge. We chose thrombin 0.2 U/ml as the fittest concentration by screening trial. All challenges underwent pretrial by CCK8 to exclude the interference of P815 cell death.