miR-23b Negatively Regulates Sepsis-Induced Inflammatory Responses by Targeting ADAM10 in Human THP-1 Monocytes

Figure 4

Effects of the interaction between miR-23b overexpression and ADAM10 inhibitor on LPS-stimulated inflammatory response and apoptosis in THP-1 cells. ADAM10 inhibitor GI254023X was applied to THP-1 cells at the concentration of 20 μM. THP-1 cells were infected by LV3-miR-23b mimics to overexpress miR-23b. Either LV3-miR-23b infection or ADAM10 inhibitor significantly decreased the production of IL-1β, IL-6, and TNF-α and apoptosis in THP-1 cells compared to the control upon LPS stimulation (500 ng/mL), while together did not significantly alleviate the inflammatory responses and apoptosis compared with LV3-miR-23b+DMSO and LV3-NC+GI254023X. Each experiment was independently repeated at least three times.

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