IGF-1R Inhibition Suppresses Cell Proliferation and Increases Radiosensitivity in Nasopharyngeal Carcinoma Cells

Figure 2

IGF-I promotes cell proliferation by activating pIGF-1R, which can be reversed by linsitinib. (a) Serum-starved CNE-2 and SUNE-1 cells were incubated with increasing concentration of IGF-I, and the cell growth was determined after 72 h treatment by MTT assay. (b) The serum-starved CNE-2 and SUNE-1 cells were cultured overnight and then treated with IGF-1 (10 ng/ml) for 1 h. The cell lysates were harvested and subjected to Western blotting. (c) The serum-starved cells were pretreated with or without linsitinib (Lin., 0.4 μM) for 2 h followed by IGF-I (10 ng/ml) stimulation for 72 h. Then, cell growth was determined by MTT assay. (d) The serum-starved cells were pretreated with or without linsitinib (Lin., 0.4 μM) for 2 h followed by indicated IGF-I stimulation for 1 h, and then, cell lysates were subjected to Western blotting. ; .

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