Research Article

PPAR γ/Nnat/NF-κB Axis Involved in Promoting Effects of Adiponectin on Preadipocyte Differentiation

Figure 1

Expression levels of APN and Nnat following coinfection of 3T3-L1 cells with adenovirus holding Ad-apM1 and Ad-nnat-shRNA. For evaluating the compatibility of overexpression of 3T3-L1 mouse cells expressing the Ad-apM1 and Ad-nnat-shRNA, western blotting was used to detect the expression levels of Nnat and APN in 3T3-L1 cells after coinfecting with adenoviruses, respectively, holding Ad-apM1 and Ad-nnat-shRNA (APN + Nnat), or adenoviruses holding empty plasmids (Ctrl-av) only for 48 h. (a) Expression levels of human APN and mouse Nnat in groups of Ctrl-av and APN+Nnat. (b) The relative band intensity of APN and Nnat in each group. Data are presented as the error of the mean for six independent experiments. vs. the Ctrl-av group.
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