Suberoylanilide Hydroxamic Acid Attenuates Autoimmune Arthritis by Suppressing Th17 Cells through NR1D1 Inhibition

Figure 7

Target molecule of SAHA. (a) Confocal microscopy of spleen cryosections, stained for DAPI, CD4, and NR1D1 (original magnification, 400x). The mean fluorescence intensities are shown (). (b) Total splenocytes were cultured under Treg-polarizing conditions for 3 days. The cells were converted to Th17-polarizing conditions with SAHA for 2 days. NR1D1 mRNA expression was analyzed. (c) Total splenocytes were cultured under Treg-polarizing conditions for 3 days. The cells were converted to Th17-polarizing conditions with SAHA for 3 days. NR1D1 expression was confirmed in cells cultured for a total of 6 days by confocal microscopy. Relative bar charts are shown. (d) Total splenocytes were isolated from splenocytes and cultured with SAHA or SR8278 under Th17-polarizing conditions for 3 days, and Th17 cell populations were analyzed by flow cytometry. , , and .

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