Figure 3: Histological characteristics from the prophylactic regimen. Representative H&E and Safranin-O-stained slides from the PBS (a–c), 1 μg of RvE1 (d-f), and anti-TNF groups (g-i). The sections clearly show (a, b, d, e) the influx of inflammatory cells in the ankle joints of CIA mice. Moreover, bone resorption and cartilage destruction were also observed. In the Safranin-O-stained slides (c, f), proteoglycan depletion was noted. Original magnification of 10x, 20x, and 50x. Images from the anti-TNF-treated mice stained with H&E (g, h) and Safranin-O (I) illustrating decreased synovial inflammation, cartilage destruction, and bone erosion and normal proteoglycan layer.