Escherichia coli Nissle 1917 Protects Intestinal Barrier Function by Inhibiting NF-κB-Mediated Activation of the MLCK-P-MLC Signaling Pathway

Figure 2

EcN^sup attenuated the injury of intestinal barrier function induced by TNF-α/IFN-γ. (a) Caco-2 monolayers were incubated with or without 10 ng/mL TNF-α and 10 ng/mL IFN-γ in the presence or absence of 100, 300, and 600 ng/mL EcN^sup for 48 h. EcN^sup significantly attenuated the increase of FD-4 flux induced by TNF-α/IFN-γ treatment. (b) Caco-2 monolayers were incubated with or without 10 ng/mL TNF-α and 10 ng/mL IFN-γ in the presence or absence of 100 ng/mL EcN^sup for 48 h. EcN^sup significantly attenuated TEER reduction induced by TNF-α/IFN-γ treatment since 12 h. (c) Caco-2 monolayers were treated as described in (b). EcN^sup significantly attenuated the increase of FD-4 flux induced by TNF-α/IFN-γ treatment since 12 h. Results were expressed as the (). vs. control. ^# vs. TNF-α/IFN-γ.

(a)

(b)

(c)