Escherichia coli Nissle 1917 Protects Intestinal Barrier Function by Inhibiting NF-κB-Mediated Activation of the MLCK-P-MLC Signaling Pathway

Figure 3

EcN^sup reversed the downregulation of TJ proteins induced by TNF-α/IFN-γ at both mRNA and protein levels. The total protein or RNA of monolayers was harvested after incubation for 48 h with or without 10 ng/mL TNF-α and 10 ng/mL IFN-γ in the presence or absence of 100 ng/mL EcN^sup. The mRNA and protein level of ZO-1, Claudin-1, Claudin-2, and Occludin were tested by (a) RT-qPCR and (b) western blot, respectively. Results were expressed as the (). vs. control. ^# vs. TNF-α/IFN-γ.

(a)

(b)