Dynamic Changes of Th1 Cytokines and the Clinical Significance of the IFN-γ/TNF-α Ratio in Acute Brucellosis

Figure 4

The percentage analysis of serum Th1 cells in different stages at 0, 6, and 12 months. (a) PBMCs were collected in the peripheral blood of new patients and follow-up subjects. The cells were fixed and permeabilized, followed by staining with PerCP-Cy™5.5 mouse anti-human CD3, APC-HT mouse anti-human CD4, and intracellular staining with BV421 mouse anti-human IFN-γ. Next, the frequency of CD3⁺CD4⁺IFN-γ⁺ T cells (Th1) was calculated with pictures showing IFN-γ in different stages. (b) Dynamic changes in Th1 cell numbers at 0, 6, and 12 months. (c) Percentage of Th1 cells in different groups. (A) Data analysis of the percentages of Th1 cells in different groups. The data are shown as the mean values of individual participants from two separate experiments. The horizontal lines indicate the median values of the different groups. A statistically significant difference was found between the patients and HC groups (). (B) Statistically significant differences could be observed in the percentages of Th1 cells between 0 and 6 months later in these two groups (). (C) Statistically significant difference could be observed on the percentages of Th1 cells between 0 and 12 months later in the two groups (). (D) A statistically significant difference was observed in the percentages of Th1 between 6 months and 12 months in the two groups (). Abbreviations: Th1: T-helper type 1; IFN-γ: gamma-interferon; HC: healthy controls.

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