Ethanol Extract of Sesamum indicum Linn. Inhibits FcεRI-Mediated Allergic Reaction via Regulation of Lyn/Syk and Fyn Signaling Pathways in Rat Basophilic Leukemic RBL-2H3 Mast Cells

Figure 5

Effect of SIE on the Ag/IgE-induced passive cutaneous anaphylaxis model. ICR mice were intradermally administered DNP-IgE (4 μg/mL) via the ear for 24 hr and were then were orally administered SIE (250 and/or 500 mg/mL) and dexamethasone (10 mg/mL). One hour later, DNP-HSA (300 μg/mL) containing 1% Evans blue was intravenously injected into their tail veins for 1 hr. (a, b) The extravasated dye in the ears was analyzed using the procedure described in Material and Methods. (c) The average numbers of mast cells per field. Paraffin-embedded skin sections were prepared and stained with (d) toluidine blue and (e) H&E staining.  μm. Results are expressed as of at least five independent experimental results that were tested by analysis of variance with Bonferroni’s post hoc testing; versus the control group; versus the IgE/Ag-treated group.