PG102 Upregulates IL-37 through p38, ERK, and Smad3 Pathways in HaCaT Keratinocytes

Figure 4

PG102 increases IL-37 expression through p38 and ERK/Smad3 axis. (a) HaCaT cells were treated with PG102 at different concentrations for 30 minutes, and the protein levels of phospho-p38 and p38 were analyzed by Western blot analysis. (b) Cells were pretreated with SB203580 (20 μM) for 1 hour, followed by treatment with PG102 (1 mg/mL) for 48 hours. The protein levels of IL-37, phospho-Smad3, and Smad3 were analyzed by Western blot analysis. (c) Cells were treated with PG102 at different concentrations for 30 minutes, and the protein levels of phospho-ERK1/2 and ERK1/2 were analyzed by Western blot analysis. (d) Cells were pretreated with U0126 (5 μM) for 1 hour, followed by treatment with PG102 (1 mg/mL) for 48 hours. The protein levels of IL-37, phospho-Smad3, and Smad3 were analyzed by Western blot analysis. Representative results from three independent experiments are shown.

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