Prior Exposure to Immunosuppressors Sensitizes Retinal Microglia and Accelerates Optic Nerve Regeneration in Zebrafish

Figure 6

Multiple intravitreal injections of dex or clodronate liposomes accelerate tectal reinnervation after optic nerve injury. (a) Schematic representation of the experimental setup. Intravitreal injection of dex or clodronate liposomes (or saline) is performed three times, at days 0, 2, and 4, and ONC is performed at day 1. Tectal reinnervation is assessed at day 7 (i.e., 6 dpi). (b-e) Repeated intravitreal injections of dex induce significantly higher tectal reinnervation in the dex + ONC group as compared to the saline + ONC group (-test, ). (f-i) Similarly, representative pictures and quantification of tectal reinnervation show a significant increase after treatment with clodronate liposomes compared to saline + ONC fish (-test, ). Scale bars: 50 μm. Values represent . -14 (b-e), (f-i).

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