Prior Exposure to Immunosuppressors Sensitizes Retinal Microglia and Accelerates Optic Nerve Regeneration in Zebrafish

Figure 8

Local treatment with dex increases the retinal cytokine expression. (a) Schematic representation of the experimental setup. Intravitreal injection (IVT) of dex (or saline) is performed three times, at days 0, 2, and 4. ONC is performed at day 1. Retinal cytokine expression is assessed via qRT-PCR at day 2 (before the injection) and at day 7. (b-i) Quantification reveals a significant increase in the expression of IL-1β, TNF-α, and LIF at both days 2 and day 7 upon dex + ONC treatment compared to the saline + ONC group. For IL-6 and IL-10, the increase is significant only at day 7 or day 2, respectively. IL-13 shows a significantly reduced expression at day 7 in dex + ONC fish. The expression levels of IL-4 and CNTF remain unchanged (-tests, , , , and ). Values represent . -7.

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