Electronegative LDL from Rabbits Fed with Atherogenic Diet Is Highly Proinflammatory

Figure 6

Effects of LDL(-) and Cu-ox-nLDL on the activation of IκB, p38, ERK1/2, and JNK in THP-1 macrophages. (a) Cells were treated with 20 μg/ml of LDL(-) or Cu-ox-nLDL for 2 h, and then levels of phosphorylated IκB (p-IκB), total IκB, p-p38, total p38, p-ERK1/2, total ERK1/2, p-JNK, and total JNK were determined by Western blotting. β-Actin was used as a loading control. (b–e) Protein levels were quantified using ImageJ software, and relative levels of p-IκB/total IκB (b), p-p38/total p38 (c), p-ERK1/2/total ERK1/2 (d), and p-JNK/total JNK (e) are expressed relative to PBS-treated cells (). Values are the of three independent experiments. , compared to corresponding PBS-treated and Cu-ox-nLDL-treated cells.

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