Isorhamnetin Inhibits Liver Fibrosis by Reducing Autophagy and Inhibiting Extracellular Matrix Formation via the TGF-β1/Smad3 and TGF-β1/p38 MAPK Pathways
IH attenuated ECM accumulation in livers. (a) qPCR was used to determine the mRNA expression of PPAR-γ, α-SMA, Col-1α1, Col-1α2, MMP-2, and TIMP1 in liver tissues. Results are given as fold change over the control (vehicle or sham) group. (b) Western blotting was used to detect the protein expression of PPAR-γ, α-SMA, Col-1, MMP-2, and TIMP1 in liver tissues in mice of each group. (c) Immunohistochemical staining was performed to analyze the expression of α-SMA and Col-1 in liver sections (brown indicates positive staining, original magnification: ×200, scale bar: 100 μm). Data are presented as (, compared to the vehicle (sham) group, compared to the CCl4 (BDL) group, and compared to the CCl4 (BDL)+IH 10 mg/kg group).
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