Mediators of Inflammation / 2019 / Article / Fig 6

Research Article

Isorhamnetin Inhibits Liver Fibrosis by Reducing Autophagy and Inhibiting Extracellular Matrix Formation via the TGF-β1/Smad3 and TGF-β1/p38 MAPK Pathways

Figure 6

IH regulated the TGF-β1-mediated Smad3 and p38 MAPK signaling pathways. (a) The serum level of TGF-β1 was detected by ELISA. (b) The mRNA expression of TGF-β1 in the liver was analyzed by qPCR. Results are given as fold change over the control (vehicle or sham) group. (c) The protein expression of TGF-β1, p-Smad3, and p-p38 MAPK was analyzed by western blotting. (d) The protein expression of TGF-β1, p-Smad3, and p-p38 MAPK in liver sections was analyzed using immunohistochemical staining (brown indicates positive staining, original magnification: ×200 and ×400, scale bar: 100 μm). Data are presented as (, compared to the vehicle (sham) group, compared to the CCl4 (BDL) group, and compared to the CCl4 (BDL)+IH 10 mg/kg group).
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