Isorhamnetin Inhibits Liver Fibrosis by Reducing Autophagy and Inhibiting Extracellular Matrix Formation via the TGF-β1/Smad3 and TGF-β1/p38 MAPK Pathways

Table 1

Nucleotide sequences of the primers used in the qPCR assays.

Gene	Amplicon size	Primer sequence	Position
β-Actin	154	F: 5-GGCTGTATTCCCCTCCATCG-3	84-103
		R: 5-CCAGTTGGTAACAATGCCATGT-3	237-216
α-SMA	104	F: 5-CCCAGACATCAGGGAGTAATGG-3	118-139
		R: 5-TCTATCGGATACTTCAGCGTCA-3	221-200
F4/80	127	F: 5-CTGCACCTGTAAACGAGGCTT-3	168-188
		R: 5-GCAGACTGAGTTAGGACCACAA-3	294-273
Col-1α1	91	F: 5-GCTCCTCTTAGGGGCCACT-3	30-48
		R: 5-ATTGGGGACCCTTAGGCCAT-3	120-101
Col-1α2	222	F: 5-TCGTGCCTAGCAACATGCC-3	49-67
		R: 5-TTTGTCAGAATACTGAGCAGCAA-3	270-248
TIMP1	108	F: 5-CGAGACCACCTTATACCAGCG-3	256-276
		R: 5-ATGACTGGGGTGTAGGCGTA-3	335-317
MMP-2	75	F: 5-GGACAAGTGGTCCGCGTAAA-3	652-671
		R: 5-CCGACCGTTGAACAGGAAGG-3	726-707
Beclin-1	149	F: 5-ATGGAGGGGTCTAAGGCGTC-3	1-20
		R: 5-TGGGCTGTGGTAAGTAATGGA-3	149-129
LC3	322	F: 5-GACCGCTGTAAGGAGGTGC-3	43-61
		R: 5-AGAAGCCGAAGGTTTCTTGGG-3	364-344
TGF-β1	91	F: 5-CCACCTGCAAGACCATCGAC-3	92-111
		R: 5-CTGGCGAGCCTTAGTTTGGAC-3	182-162
PPAR-γ	121	F: 5-GGAAGACCACTCGCATTCCTT-3	69-89
		R: 5-GTAATCAGCAACCATTGGGTCA-3	189-168