Inhibition of Cerebral High-Mobility Group Box 1 Protein Attenuates Multiple Organ Damage and Improves T Cell-Mediated Immunity in Septic Rats

Figure 1

Intracerebroventricular (ICV) injection of BoxA ameliorated multiple organ damage under sepsis exposure. (a–e, h, i) Serum biochemical parameters, including creatine kinase (CK), CK-MB, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholylglycine (CG), blood urea nitrogen (BUN), and creatinine (Cr), were measured using a HITACHI 7600 biochemical analyser as these parameters reflect multiple organ dysfunction. Serum samples were collected at 24 h after cecal ligation and puncture (CLP) surgery. BoxA solution (1 μg or 10 μg) was injected into the left lateral ventricle immediately after operation. (f, g) The wet-to-dry ratio (W/D) and myeloperoxidase (MPO) activity of pulmonary tissues were quantified by weighing and using an ELISA kit, respectively. Lungs were harvested and weighed at 24 h after CLP surgery. (Each bar represents the of three independent experiments, ; vs. the sham group; vs. the sepsis group; vs. the sepsis plus 1 μg BoxA group; vs. the sepsis plus 1 μg BoxA group.)

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