RIPK2-Mediated Autophagy and Negatively Regulated ROS-NLRP3 Inflammasome Signaling in GMCs Stimulated with High Glucose

Figure 3

LC3 expression in HG-induced SV cells. LC3II/I expression increased in the HG group in a concentration-dependent manner at both 12 h and 72 h, especially for 72 h (a, b). In addition, HG caused an increase in LC3II/I in a time-dependent manner, detected by western blotting (d, e). Representative confocal microscopy images of tfLC3 puncta in SVs stained for autophagosomes (green) and autolysosomes (red) for colocalization as an indicator of autophagy. We observed that autophagy increased in the HG group in a concentration-dependent and time-dependent manner (c, f). .

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