Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes

Figure 1

Altered intracellular protein expression caused by gain or loss of DICER1 function for 48 h in human synoviocytes SW982. (a) Representative Western blotting image of DICER1 expression after transfected with 10 nM DICER1 siRNAs 1, 2, and 3 or multiple combinations. (b) Representative Western blotting image of DICER1 expression after transfected with 1, 2, and 5 μg overexpression vector per well in 6-well plates. (c) Western blotting results of DICER1, TLR3, COX2, MMP3, MMP8, MMP13, and GAPDH protein expression after DICER1 RNAi. (d) Western blotting results of DICER1, TLR3, COX2, MMP3, MMP8, MMP13, and GAPDH protein expression after DICER1 overexpression. (A) Representative images; (B) quantitative results. Bar: from three independent cell experiments, using the Mann-Whitney test.

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