The Differential Expression of Mitochondrial Function-Associated Proteins and Antioxidant Enzymes during Bovine Herpesvirus 1 Infection: A Potential Mechanism for Virus Infection-Induced Oxidative Mitochondrial Dysfunction

Figure 2

BoHV-1 infection affected the expression of certain components in mitochondrial RC complexes. (a) MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 at an MOI of 1 for 2, 4, 8, and 16 hours. The cell lysates were then prepared for Western blots to detect NDUFB8 for complex I, SDHB for complex II, MTCO1 for complex IV, UQCRC2 for complex III, and ATP5A for complex V, using OXPHOS antibody cocktail (Abcam; ab110413, 1 : 2000). Data shown are representative of three independent experiments. (b) The relative band intensity was analyzed with software ImageJ, and each analysis was compared with that of uninfected control which was arbitrarily set as 100%. Data are means of three independent experiments. Significance was assessed with the Student -test ().

(a)

(b)