The Differential Expression of Mitochondrial Function-Associated Proteins and Antioxidant Enzymes during Bovine Herpesvirus 1 Infection: A Potential Mechanism for Virus Infection-Induced Oxidative Mitochondrial Dysfunction

Figure 5

The effects of virus infection on the expression of NRF1, NRF2, and TFAM. MDBK cells in 60 mm dishes were mock infected or infected with BoHV-1 at an MOI of 1 for 2, 4, 8, and 16 hours. The cell lysates were then prepared for Western blots to detect the expression of NRF1 (a), NRF2 (b), and TFAM (c) using NRF1 antibody (GeneTex; # PA5-40912, 1 : 1000), NRF2 antibody (Abcam; # ab137550, 1 : 500), and TFAM polyclonal antibody (Thermo Fisher Scientific; # PA5-68789, 1 : 1000). (d) The relative band intensity was analyzed with software ImageJ, and each analysis was compared with that of uninfected control which was arbitrarily set as 100%. Data are means of three independent experiments. Significance was assessed with the Student -test ().

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