Trypanosoma cruzi Mexican Strains Differentially Modulate Surface Markers and Cytokine Production in Bone Marrow-Derived Dendritic Cells from C57BL/6 and BALB/c Mice

Figure 4

Percentage of variation in the levels of pro-and anti-inflammatory cytokines and CCL-2 chemokine in the supernatant of BMDCs of BALB/c and C57BL/6 mice. The variation of cytokines was calculated by determination of percentage of increment or reduction in cytokines produced by T. cruzi-infected BMDCs previously stimulated with LPS and those maintained only with LPS (5 μg/mL). (f, h) Representation of the radar graph of the cytokine pattern in the dendritic cell culture supernatant. The lines highlight the change in cytokine levels in dendritic cells infected with different strains (MOI 2 : 1) in relation to the medium only with dendritic cells. (g, i) The pattern of cytokines in the dendritic cell culture supernatant previously stimulated with LPS. Data were obtained by calculating the ratio between T. cruzi-infected cells and their respective control. Statistical analysis, when applicable, was performed with the Kruskal-Wallis test with Dunn’s posttest, where .

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