Trypanosoma cruzi Mexican Strains Differentially Modulate Surface Markers and Cytokine Production in Bone Marrow-Derived Dendritic Cells from C57BL/6 and BALB/c Mice

Figure 6

Expression of MHC-II, costimulatory molecules, and toll-like receptors in BMDCs after in vitro T. cruzi infection. The expression of molecules was evaluated by flow cytometry and represented as a variation of the mean intensity of fluorescence (a) MHC-II, (b) CD80, (c) CD86, (d) TLR2, and (e) TLR4. (f, g) Representation of the cytokine secretion pattern in BMDC culture supernatant. The lines highlight the change in cytokine levels in LPS-stimulated BMDCs and infected with different strains of T. cruzi (MOI 2 : 1) in relation to uninfected LPS-stimulated BMDCs. Statistical analysis, when applicable, was performed with the Kruskal-Wallis test with Dunn’s posttest, where .

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