Th1/17-Biased Inflammatory Environment Associated with COPD Alters the Response of Airway Epithelial Cells to Viral and Bacterial Stimuli
Table 1
Sequence of primers used for qRT-PCR.
Genes
Sequence ID
Forward primers (5-3)
Reverse primers (5-3)
Product size (bp)
HPRT1
NM_000194.3
CAAAGATGGTCAAGGTCGCA
TCAAATCCAACAAAGTCTGGCT
82
CXCL8
NM_000584.4
TCAGAGACAGCAGAGCACAC
ACACAGTGAGATGGTTCCTTCC
76
IL6
NM_000600.5
AACCTGAACCTTCCAAAGATGG
TCTGGCTTGTTCCTCACTACT
159
IL1B
XM_017003988.2
GGACAGGATATGGAGCAACAAGTGG
TTCAACACGCAGGACAGGTACAGAT
125
IFNB1
NM_002176.4
GTCACTGTGCCTGGACCATA
AATTGTCCAGTCCCAGAGGC
122
TNF
NM_000594.4
TCAGCAAGGACAGCAGAGGA
GTCAGTATGTGAGAGGAAGAGAACC
128
TLR3
NM_003265.2
GCTCTGGAAACACGCAAACC
CTCGTCAAAGCCGTTGGACT
97
TLR4
NM_138554.5
TGGTGTGTCGGTCCTCAGTGT
GCAGCCAGCAAGAAGCATCAG
91
TLR7
NM_016562.4
TCTTGCCTTCTGGAGTTTTTG
CAGTGGTCAGTTGGTTGTGG
150
IFIH1
NM_022168.4
GGAGTCAAAGCCCACCATCT
AGACCTTCTTCTGCCACTGTG
146
DDX58
NM_014314.4
GCCTTGGCATGTTACACAGC
TTTGGCTTGGGATGTGGTCT
110
TLR: toll-like receptor; IFIH1: interferon-induced helicase C domain-containing protein 1, known as MDA5; DDX58: DExD/H-box helicase 58 (known as RIG-I).
