Mdivi-1 Protects CD4⁺ T Cells against Apoptosis via Balancing Mitochondrial Fusion-Fission and Preventing the Induction of Endoplasmic Reticulum Stress in Sepsis

Figure 5

Mdivi-1 reestablished the mitochondrial fusion-fission balance in the CLP mice. Mice were pretreated with Mdivi-1 (20 mg/kg) before undergoing CLP surgery and were sacrificed at 24 h after CLP surgery. (a) Western blot assay and quantitative analysis of mitochondrial and cytosolic MFN2, OPA1, and Drp1 expression. (b) Western blot assay and quantitative analysis of p-Drp1 expression. (c) Micrographs of mitochondria observed using a transmission electron microscopy and quantitative analysis of the average mitochondrial area in five CD4⁺ T cells (magnification: 10000x). Scale bars, 0.5 μm. The results are shown as the ; . vs. the sham group (, ); vs. the CLP group (, ).