Mediators of Inflammation / 2019 / Article / Fig 2

Research Article

Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells

Figure 2

Rab26 regulates LPS-induced imbalance of β2-AR and TLR4. (a) Effect of Rab26 on the cell surface expression of proteins in HPMECs. HPMECs were transfected with DsRed-tagged Rab26 plasmids for 72 h and treated with LPS (1 μg/mL) for 6 h; then, Western blot analyzed the membrane protein expression. The histograms show representative β2-AR, TLR4, and Na+-K+-ATPase expression and quantitative data normalized to Na+-K+-ATPase, respectively. (b) HPMECs were pretransfected with HA-tagged Rab26 plasmids for 72 h and treated with LPS (1 μg/mL) for 6 h. Flow cytometry detected the expression of cell surface receptors. The data are the (). versus the control group; ^ versus the mock group.
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