Mediators of Inflammation / 2019 / Article / Fig 4

Research Article

Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells

Figure 4

Knock-down of β2-AR and TLR4 in HPMECs. (a) The expression of membrane proteins after transfection with β2-AR siRNA and TLR siRNA. Quantification of protein levels by Western blotting. The representative blots show the expression of β2-AR (A, upper), TLR4 (A, middle), and Na+-K+-ATPase (A, lower). The histograms show the quantitative analysis of the levels of β2-AR and TLR4 normalized to Na+-K+-ATPase. (b, c) Expression of inflammatory factors (IL-6, IL-17A, IL-8, and TNF-α) after transfection with siRNA. (d) Effect of siRNA treatment on monolayer permeability. The data are the (). versus the NC; ^, versus the β2-AR siRNA group, #, versus the TLR4 siRNA group.

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