Enhanced Activity by NKCC1 and Slc26a6 Mediates Acidic pH and Cl⁻ Movement after Cardioplegia-Induced Arrest of db/db Diabetic Heart

Figure 6

Activity and protein expression of NHE1 between WT and db/db after HTK-induced arrest. (a) NHE activity was assessed by NH₄Cl pulse technique in LV cardiac strips of WT (closed black square) and db/db (closed red square) mice following cardioplegia-induced arrest. Full image of NHE activity was represented in the square box, and magnified images were obtained from the dotted line. (b) NHE activity in LV of WT after cardioplegia-induced arrest. (c) Analysis of NHE activity. The slope of pH_i was measured in NHE activity in the Na⁺-containing Regular solution. The bars represent the (). (d) Expression of NHE1 protein in LV and RV cardiac strips of WT and db/db following cardioplegia-induced arrest. (e) Analysis of band intensity of NHE1 in LV. The β-actin was used as the loading control. The bars show the ().

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