Protective Effects of Oridonin on Acute Liver Injury via Impeding Posttranslational Modifications of Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) in the Toll-Like Receptor 4 (TLR4) Signaling Pathway

Figure 1

Acetyltransferase inhibitory activity of oridonin was assessed in vivo and in vitro. (a) JS1 cells were seeded in 10 cm dishes and incubated for 24 h. The cells were pretreated with oridonin (5, 10, or 15 μm) for 3 h before adding 1 mM TSA. The cells were harvested after 4 h of TSA treatment. Western blot showed that oridonin decreased acetylation of H3, H4, and α-tubulin induced by TSA. The inhibitory effect of oridonin was obvious when the concentration was 15 μm. (b) There was a substantial rise in the overall pan-acetylation level, especially expression of acetyl-H3, acetyl-H4, and acetyl-α-tubulin in LPS/D-Gal-induced ALI. The overall pan-acetylation level reduced in a concentration-dependent way upon oridonin treatment. (c) Band intensity in (a) was quantified by ImageJ. ^#, ^## vs. control group; , vs. TSA treatment group.

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