A Benzenediamine Analog FC-99 Drives M2 Macrophage Polarization and Alleviates Lipopolysaccharide- (LPS-) Induced Liver Injury

Figure 3

Induction of FC-99 on M2 polarization was independent of STAT6. (a–c) RAW264.7 cells were cultured with IL-4 (10 ng/ml) or FC-99 (50 μM) for the indicated time points; levels of STAT6 phosphorylation (p-STAT6), total STAT6 (t-STAT6), and GAPDH were tested by Western blot. (b) and (c) were the relative intensity analysis of p-STAT6 using STAT6 as the internal control. (d) The silencing effect of siRNA for STAT6 was detected using Q-PCR. Data were presented as the . , , and vs. the indicated group. (e) RAW264.7 cells were transfected with control siRNA (si-NC) or siRNA targeting STAT6 (si-STAT6) for 48 h, and then cells were treated with FC-99 (50 μM), IL-4 (10 ng/ml), or both, respectively, for another 24 h. The mRNA level of CD206 was measured by Q-PCR. Data were presented as the . and vs. Con; vs. the indicated group.

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