A Benzenediamine Analog FC-99 Drives M2 Macrophage Polarization and Alleviates Lipopolysaccharide- (LPS-) Induced Liver Injury

Figure 4

FC-99 mediated M2 macrophage polarization via PPAR-γ. (a, b) RAW264.7 cells were pretreated with GW9662 at 1 μM for 2 h before IL-4 or FC-99 treatment for another 24 h, and the mRNA expression of CD206 was measured by Q-PCR. (c, d) RAW264.7 cells were treated with FC-99 (50 μM) for 24 h, the expression of PPAR-γ in the mRNA level was tested by Q-PCR (c), and the protein level in cell extracts (1 mg protein) was assessed by ELISA (d). Data were presented as the . , , and vs. the indicated group.

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