Cx43 Inhibition Attenuates Sepsis-Induced Intestinal Injury via Downregulating ROS Transfer and the Activation of the JNK1/Sirt1/FoxO3a Signaling Pathway

Figure 4

ROS inhibition improved LPS-induced IEC-6 injuries in vitro and CLP-induced intestinal injuries in vivo. (a) 18-α-GA (10 μM for 1 hour), oleamide (25 μM for 1 hour), and NAC (10 mM for 1 hour) pretreatment attenuated ROS generation and distribution between the neighboring IEC-6 cells. (b) Survival rate of IEC-6 cells pretreated with LPS (10 μg/ml for 24 hours) using CCK-8 assay. (c–e) Levels of LDH, DAO, and iFABP in supernatants. In (a–e), data are shown as , ; vs. the control group and vs. the LPS group. (f) Small intestine tissue slices were stained with H&E. Rats were intraperitoneally pretreated with NAC (200 mg/kg) for 1 hour before CLP surgery. (g) The histopathological score was estimated according to Chiu’s standard. (h–j) Levels of LDH, DAO, and iFABP in small intestine tissues. (k–m) Levels of LDH, DAO, and iFABP in serum. In (f–m), data are shown as , -8 for each group; vs. the Sham group and vs. the CLP group.

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