Cx43 Inhibition Attenuates Sepsis-Induced Intestinal Injury via Downregulating ROS Transfer and the Activation of the JNK1/Sirt1/FoxO3a Signaling Pathway

Figure 5

ROS regulated the activation of the JNK1/Sirt1/FoxO3a signaling pathway. (a) Effects of NAC (intraperitoneally, 200 mg/kg for 1 hour), sp600125 (intraperitoneally, 30 mg/kg for 1 hour), and nicotinamide (intraperitoneally, 120 mg/kg/day for 3 days) pretreatment before CLP surgery on JNK1, Sirt1, FoxO3a, Bim, and Puma expression. (b) Small intestine tissue slices were stained with H&E. Rats were intraperitoneally pretreated with NAC, sp600125, and nicotinamide before CLP surgery. (c) The histopathological score was estimated according to Chiu’s standard. (d) Levels of LDH, DAO, and iFABP in small intestine tissues. (e) Levels of LDH, DAO, and iFABP in serum. In (a–e), data are shown as , for each group; vs. the Sham group and vs. the CLP group. (f) Survival rate of IEC-6 cells pretreated with NAC (10 mM for 1 hour), sp600125 (20 μM for 24 hours), and nicotinamide (20 mM for 24 hours) before LPS (10 μg/ml for 24 hours) exposure, using CCK-8 assay. (g) Levels of LDH, DAO, and iFABP in supernatants. In (f–g), data are shown as , -5; vs. the control group and vs. the LPS group.

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