Cx43 Inhibition Attenuates Sepsis-Induced Intestinal Injury via Downregulating ROS Transfer and the Activation of the JNK1/Sirt1/FoxO3a Signaling Pathway

Figure 6

FoxO3a directly regulated Bim and Puma expression. (a) FoxO3a siRNAs (FoxO3a-siRNA1 and FoxO3a-siRNA2) attenuated FoxO3a expression in IEC-6 cells. (b) The increase of Bim luciferase activity was attenuated by FoxO3a siRNA transient transfection into IEC-6 cells with Lipofectamine 2000. (c) IEC-6 cells were pretreated with FoxO3a siRNAs (FoxO3a-siRNA1 and FoxO3a-siRNA2) and then subjected to LPS (10 μg/ml) for 24 hours. ChIP analyses were performed with antibodies against FoxO3a and primers for the Bim promoter regions. (d) The increase of Puma luciferase activity was attenuated by FoxO3a siRNA transient transfection into IEC-6 cells with Lipofectamine 2000. (c) IEC-6 cells were pretreated with FoxO3a siRNAs (FoxO3a-siRNA1 and FoxO3a-siRNA2) and then subjected to LPS (10 μg/ml) for 24 hours. ChIP analyses were performed with antibodies against FoxO3a and primers for the Puma promoter regions. Data are shown as , ; vs. the control group and vs. the LPS group.

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