Elevation of IL-6 and IL-33 Levels in Serum Associated with Lung Fibrosis and Skeletal Muscle Wasting in a Bleomycin-Induced Lung Injury Mouse Model

Figure 3

BLM-induced lung injury may cause body weight loss and muscle atrophy. (a) The body weights of experimental mice were measured every two to three days during the experimental periods. Square boxes represent the body weight change of control mice (), and dots represent the body weight change of mice with BLM-induced lung injury (). The quantitative data were expressed as the . compared with control. (b–g) Cross-sectional H&E staining of quadriceps muscle in (b, e) control mice and (c, f) mice with BLM-induced lung fibrosis. The photos represent 50x magnification in (b–d) and 200x magnification in (e–g). The quantitative analysis of the muscle bundle area to the total area in photos is shown in (d, g) (). The values of all data are averages from at least three photos with different fields of view. The muscle bundle area was measured by ImageJ software. compared with control mice. at 50x magnification and 50 μm at 200x magnification as indicated. The black arrow indicates the detached basal lamina.