Soluble CD14 Enhances the Response of Periodontal Ligament Stem Cells to Toll-Like Receptor 2 Agonists

Figure 5

Response of hPDLSCs to different sCD14 concentrations. Primary hPDLSCs were stimulated for 24 hours with different sCD14 concentrations (2.5 ng/ml–250 ng/ml) in the absence of TLR-2 agonists. Untreated cells served as the control. After 24 hours of incubation, IL-6, CXCL8, and CCL2 gene expression levels (a) were measured using qPCR. The -axis shows the -fold expression of the target genes compared to the unstimulated control. The corresponding protein levels in conditioned media (b) were determined by ELISA. All data are presented as from five independent experiments using cells from five different donors. value < 0.05 compared to the control.

(a)

(b)