Mediators of Inflammation

Research Article

Th1Th17_CM Lymphocyte Subpopulation as a Predictive Biomarker of Disease Activity in Multiple Sclerosis Patients under Dimethyl Fumarate or Fingolimod Treatment

Phenotype of T cell subpopulations by flow cytometry.


Lymphocyte subpopulations	Phenotype

T cell subsets (CD3⁺)
CD4⁺ naïve T cell	CD4⁺CCR7⁺CD45RA⁺
CD8⁺ naïve T cell	CD8⁺CCR7⁺CD45RA⁺
CD4⁺ central memory (CD4⁺ T_CM)	CD4⁺CCR7⁺CD45RA^-
Th1 central memory (Th1_CM)	CD4⁺CCR7⁺CD45RA^-CCR6^-CXCR3⁺
Th2 central memory (Th2_CM)	CD4⁺CCR7⁺CD45RA^-CCR6^-CXCR3^-
Th17 central memory (Th17_CM)	CD4⁺CCR7⁺CD45RA^-CCR6⁺CXCR3^-
Th1Th17 central memory (Th1Th17_CM)	CD4⁺CCR7⁺CD45RA^-CCR6⁺CXCR3⁺
CD8+ central memory T cell (CD8⁺ T_CM)	CD8⁺CCR7⁺CD45RA^-
CD4⁺ effector memory T cell (CD4⁺ T_EM)	CD4⁺CCR7^-CD45RA^-
Th1 effector memory (Th1_EM)	CD4⁺CCR7^-CD45RA^-CCR6^-CXCR3⁺
Th2 effector memory (Th2_EM)	CD4⁺CCR7^-CD45RA^-CCR6^-CXCR3^-
Th17 effector memory (Th17_EM)	CD4⁺CCR7^-CD45RA^-CCR6⁺CXCR3^-
Th1Th17 effector memory (Th1Th17_EM)	CD4⁺CCR7^-CD45RA^-CCR6⁺CXCR3⁺
CD8⁺ effector memory T cell (CD8⁺ T_EM)	CD8⁺CCR7^-CD45RA^-
Terminal differentiated effector memory CD4⁺ T cell (T_EMRA)	CD4⁺CCR7^-CD45RA⁺
Terminal differentiated effector memory CD8⁺ T cell (T_EMRA)	CD8⁺CCR7^-CD45RA⁺
Double negative T cell	CD4^-CD8^-
Double positive T cell	CD4⁺CD8⁺