In Vivo Effects of Neostigmine and Physostigmine on Neutrophil Functions and Evaluation of Acetylcholinesterase and Butyrylcholinesterase as Inflammatory Markers during Experimental Sepsis in Rats

Figures 3

(a, b) Effects of physostigmine and neostigmine treatment on ROS production of PMNs during CLP-induced sepsis in rats. (a) ROS production in the sham group () did not change significantly during the entire experiment (). In the control (), physostigmine () and neostigmine () groups’ ROS production increased significantly at , 15, and 20 h, respectively (). (b) ROS production by PMNs following in vitro stimulation with fMLP increased significantly at , 15, and 20 h in the control group (). The animals treated with either physostigmine or neostigmine showed decreased levels of ROS production, which reached significance at (^§). The comparison between the animals treated with either physostigmine or neostigmine revealed no significant differences in ROS production over the course of the experiment. The data are presented as the . versus ; ^§ versus the control, analysis of variance followed by Bonferroni’s multiple comparisons test.

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(b)