In Vivo Effects of Neostigmine and Physostigmine on Neutrophil Functions and Evaluation of Acetylcholinesterase and Butyrylcholinesterase as Inflammatory Markers during Experimental Sepsis in Rats

Figures 4

(a, b) Effects of physostigmine and neostigmine treatment on CD11b and CD62l expression levels of PMNs during CLP-induced sepsis in rats. (a) In the sham group (), CD62l expression was significantly higher 6 h after beginning the experiment (). In the control () and treatment groups ( neostigmine, physostigmine), no significant differences in CD62l expression were observed over the course of the experiment. (b) CD11b expression in the sham group () did not change significantly during the entire experiment (). CLP-induced sepsis led to a strong increase in CD11b expression, which reached significance at and 15-20 h in the control group (, ). In the physostigmine () and neostigmine () groups, CD11b expression increased, reaching significance at . At -20 h, CD11b expression was significantly reduced in the animals treated with physostigmine or neostigmine compared with the control group (^§). The data are presented as the . versus ; ^§ versus the control, Friedman’s two-way ANOVA and Kruskal-Wallis test followed by Bonferroni’s pairwise comparison test.

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