In Vivo Effects of Neostigmine and Physostigmine on Neutrophil Functions and Evaluation of Acetylcholinesterase and Butyrylcholinesterase as Inflammatory Markers during Experimental Sepsis in Rats

Figures 6

(a, b) Relative AChE and relative BChE activity during CLP-induced sepsis in rats (results for physostigmine- and neostigmine-treated animals are combined as “treatment group”). Relative activity for the given time point was calculated as the ratio between the measured absolute enzyme activity for the given time point and the enzyme activity at . (a) In the control group (), AChE activity decreased versus baseline, being significant at , 15, 20, and 24 h (). The sham group () did not show any differences in AChE activity during the observation time of 24 h. In the treatment groups (physostigmine and neostigmine, ), AChE activity significantly decreased at and 24 h (^§). (b) There were no significant differences of BChE activity until in any group. At , BChE activity was significantly decreased in the control group (, ). Box plots in panels represent medians with 25% and 75% percentiles; error bars are minimum and maximum values. versus in the placebo group. ^§ versus in the treatment group, Friedman’s two-way ANOVA followed by Bonferroni’s pairwise comparison test.

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