Potential Role of Caveolin-1 in Regulating the Function of Endothelial Progenitor Cells from Experimental MODS Model

Figure 2

Characterization of endothelial progenitor cells (EPCs). (a) On day 7, after culture, EPCs were stained with antibodies against KDR, CD133, CD34, and CD45 and analyzed by flow cytometry. More than 99% of the cultured cells were positive for KDR, CD133, CD34, and CD45. (b) Ultrastructure of EPCs by electron microscopy. Black arrow shows a Weibel-Palade body, which is a structural characteristic of EPCs. Magnification: (left) ×5000, (right) ×30000. (c) Endothelial progenitor cells were labeled with Dil-ac-LDL and stained with FITC-labeled BS-lectin. Double-positive cells were considered EPCs. Magnification: ×400.

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