Could Increased Expression of Hsp27, an “Anti-Inflammatory” Chaperone, Contribute to the Monocyte-Derived Dendritic Cell Bias towards Tolerance Induction in Breast Cancer Patients?

Figure 1

Mo-DCs from a breast cancer patient show lower frequency of maturation surface markers than those from healthy donors. (a) Box plots represent the frequency of DC maturation markers. Monocytes were cultivated for 7 days in the presence of GM-CSF and IL-4 (50 ng/ml each) to induce the differentiation of immature Mo-DCs; cell maturation was induced by TNF-α (50 ng/mL) during the last 2 days of culture. On day 7 of culture, Mo-DCs were harvested and labeled with monoclonal antibodies specific to the molecules CD1a, CD11c, CD80, CD86, CD40, HLA-DR, and CCR-7. (b) Allogeneic T lymphocytes previously labeled with CFSE were cocultured with Mo-DCs (from healthy donors or from patients). On day 4 of coculture, the cells were harvested, stained with a viability marker, acquired by flow cytometry, and an index of proliferation was determined by FlowJo software. (c, d) Concentration of IFN-γ and IL-10 in supernatants of cocultures with patients’ Mo-DC or healthy donors’ Mo-DC, determined by ELISA. The results are expressed as the . ( patients and healthy donors); a -test was used to compare patients and healthy donor groups. .

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