Could Increased Expression of Hsp27, an “Anti-Inflammatory” Chaperone, Contribute to the Monocyte-Derived Dendritic Cell Bias towards Tolerance Induction in Breast Cancer Patients?

Figure 2

Cancer cell lines (MCF-7 and SKBR3) disturbed the maturation of Mo-DCs. (a, b) Box plots represent the frequency of HLA-DR and CD11c or HLA-DR and CD86-expressing Mo-DCs from healthy donors and breast cancer patients. Monocytes were cultivated in the presence of GM-CSF and IL-4, and Mo-DCs maturation was induced by TNF-α. MCF-7 and SKBR3 breast cancer cell lines were cocultured in a transwell system with the Mo-DCs. On day 7 of culture, Mo-DCs were harvested and labelled with monoclonal antibodies for CD11c, CD86, and HLA-DR. (c) Contour plots illustrating or expression under the various conditions. (d) Proliferation index obtained in mixed lymphocyte reaction (MLR) with healthy donors’ or breast cancer patients’ Mo-DC cocultured or not with tumor cell lines, as stimulators. (e, f) IFN-γ and IL-10 concentrations in MLR supernatants. Cytokine concentrations were determined by ELISA. The results are expressed as the . ( patients and healthy donors). A -test was used to compare different groups. when compared with Mo-DC obtained from healthy donors and ^# when compared with Mo-DC from healthy donors in transwell with the respective tumor line cell.

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