Blockade of IL-17A/IL-17R Pathway Protected Mice from Sepsis-Associated Encephalopathy by Inhibition of Microglia Activation

Figure 6

(a) Primary microglial cells were collected and cultured with the purity of over 90%. Cells were stained with anti-CD11b antibody and examined by flow cytometry. (b) IL-17A or LPS induced cytokine production in microglia in vitro. Microglia cultured from CLP or sham-operated mice was pretreated with anti-IL-17R ab or isotype control and then stimulated with or without IL-17A (50 ng/ml or 100 ng/ml) or LPS (1 μg/ml). Supernatant was collected 24 h after stimulation and measured by ELISA. (c) Mean fluorescence intensity of Iba-1 in cultured microglia was determined by flow cytometry. vs. PBS control (sham group); ^# vs. PBS control (CLP group); ^† CLP vs. sham; ^$ vs. IL-17A 100 ng/ml (CLP group); ^‡ vs. IL-17A 100 ng/ml (sham group).

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