IL-10 Dampens the Th1 and Tc Activation through Modulating DC Functions in BCG Vaccination
IL-10KO mice had higher IFN γ production after BCG vaccination in the spleen. The spleen was digested with enzyme as described in Materials and Methods. The cells were stained with FITC anti-CD3Ɛ, PE anti-CD4, PerCy5.5 anti-CD8, and APC anti-IFN γ. The intracellular IFN γ-positive cells were analyzed by intracellular cytokine staining when cells were gated on CD3Ɛ, CD4, and CD8 as described previously . (a) Representative figures from FAC analysis were shown. (b) Summary of percentage of the IFN γ+CD3Ɛ+, CD4+, and CD8+ T cells in the spleen, and Student’s test was used for analysis. ,. The representative of three independent experiments () with similar results was shown.